metapone

metapone conducts pathway testing from untargeted metabolomics data using a weighted permutation test.

Key Features:

• Feature-level test integration: Requires user-supplied feature-level test results from case-control testing, regression, or other suitable feature-level tests matched to the study design.
• Weighted metabolic features: Weights are assigned to metabolic features based on how many metabolites they could potentially match to.
• Multi-mode combination: Can combine positive and negative mode results in pathway tests.

Scientific Applications:

• Untargeted metabolomics pathway analysis: Enables pathway-level interpretation of untargeted metabolomics data through weighted statistical testing.
• Multi-mode metabolomics integration: Supports combined analysis of positive and negative ionization mode data for comprehensive pathway coverage.

Methodology:

The package uses a weighted permutation test for pathway testing, where metabolic features are weighted by their potential metabolite match count.