mitology

mitology studies mitochondrial activity through high-throughput RNA-seq data by leveraging a curated collection of genes whose proteins localize to mitochondria.

Key Features:

• Mitochondrial Gene Collection: Compiles genes whose proteins localize to mitochondria, derived from MitoCarta, IMPI, MSeqDR, and Gene Ontology (genes annotated with terms related to 'mitochondri-' in their description).
• MitoCarta3.0 Pathway Implementation: Provides ready-to-use implementations of MitoCarta3.0 pathways for direct analysis.
• Pathway Reorganization: Reorganizes Reactome and Gene Ontology pathways with a specific focus on pathways related to mitochondria activity.
• Mitochondrial Pathway Analysis: Conducts mitochondrial pathway analyses using mitochondria-focused pathways, enabling single-sample assessments.
• Multi-Platform Data Support: Accommodates classical gene expression data, single-cell transcriptomics, and spatial transcriptomics.

Scientific Applications:

• Mitochondrial Activity Dissection: Enables specific exploration of mitochondrial activity through transcriptomic profiles, overcoming limitations of traditional pathway analyses where mitochondrial components are small portions of larger signaling networks.
• Targeted Mitochondrial Studies: Serves as an instrument for conducting targeted investigations of impaired mitochondrial function and its variable phenotypic expressions.
• Single-Sample Assessment: Allows mitochondrial pathway analysis at the individual sample level.

Methodology:

mitology utilizes mitochondria-focused pathways derived from a curated mitochondrial gene list to conduct mitochondrial pathway analyses on transcriptomic data.