ModCon

ModCon calculates alternative nucleotide sequences surrounding splice donor sites to either activate or repress donor usage while maintaining the same amino acid encoding.

Key Features:

• Splice donor site modification: Calculates nucleotide sequence changes surrounding splice donor sites to modulate their usage.
• Genetic code preservation: Proposed alternative nucleotide sequences encode the same amino acid as the original sequence.
• Directional modulation: Enables both activation and repression of donor site usage depending on experimental needs.

Scientific Applications:

• Cryptic splice donor site silencing: Alternative nucleotide sequences can be applied to silence cryptic splice donor sites in reporter systems.
• Cryptic splice donor site activation: Alternative nucleotide sequences can be applied to activate cryptic splice donor sites in reporter systems.
• mRNP code manipulation: Modifies splice site usage by changing the mRNP code while maintaining the genetic code.

Methodology:

Collection of functions that calculate nucleotide sequences surrounding splice donor sites.