OAtools

OAtools provides a suite of R functions to analyze gene expression experiments on the OpenArray real-time PCR platform by fitting logistic regressions to fluorescence curves to distinguish real amplification from false positives.

Key Features:

• Data Import: Supports import of OpenArray real-time PCR data.
• Amplification Detection: Fits logistic regressions to fluorescence curves to distinguish between real amplification and false positives.
• Visualization: Generates plots and a dynamic HTML report for data analysis results.

Scientific Applications:

• Gene Expression Analysis: Analyzes gene expression experiments performed on the OpenArray real-time PCR platform.
• qPCR Quality Control: Distinguishes true amplification signals from false positives in real-time PCR fluorescence data.

Methodology:

OAtools fits logistic regressions to fluorescence curves.