PoDCall

PoDCall reads files exported from QX Manager or QuantaSoft containing amplitude values from a run of ddPCR (96 well plate) and robustly sets thresholds to determine positive droplets for each channel of each individual well.

Key Features:

• Threshold Setting: Robustly sets thresholds to determine positive droplets for each channel of each individual well.
• Concentration Calculation: Calculates concentration and normalized concentration in addition to other metrics for each well.
• Result Export: Returns results as a table, optionally written to file.
• Visualization: Generates optional scatterplot and histogram plots for both channels per well, optionally written to file.

Scientific Applications:

• DNA Methylation Droplet Digital PCR: Positive droplet calling for DNA methylation droplet digital PCR experiments.
• ddPCR Data Analysis: Analysis of ddPCR amplitude data from 96 well plate experiments exported from QX Manager or QuantaSoft.

Methodology:

Reads files exported from QX Manager or QuantaSoft containing amplitude values from a run of ddPCR (96 well plate) and robustly sets thresholds to determine positive droplets for each channel of each individual well.