rifiComparative

rifiComparative compares two conditions output of rifi using half-life and mRNA at time 0 segments to enable differential transcript stability analysis.

Key Features:

• Segmentation: Segments data using the difference between half-life of both conditions and log2 fold change of mRNA at time 0 as input.
• Statistical Analysis: Provides statistics to support comparative interpretation of transcript stability differences.
• Summary Table Generation: Produces summary tables consolidating comparative results across conditions.
• Fragment Visualization: Generates visualizations of fragments identified from the segmentation process.
• Additional Plots: Produces supplementary plots for further analysis of comparative transcript stability data.

Scientific Applications:

• Differential RNA Half-life Analysis: Compares transcript half-life between two experimental conditions to identify stability changes.
• mRNA Abundance Comparison: Analyzes log2 fold change of mRNA at time 0 to assess initial abundance differences between conditions.
• Transcript Stability Profiling: Enables genome-wide comparison of RNA stability dynamics across biological conditions.

Methodology:

rifiComparative takes as input the output of rifi from two different conditions and performs segmentation using the difference between half-life of both conditions and log2 fold change of mRNA at time 0.