SanityR

SanityR provides an R/Bioconductor interface to the Sanity model for gene expression analysis.

Key Features:

• Bayesian Normalization: Implements a Bayesian normalization procedure derived from first principles.
• Direct UMI Processing: Estimates expression values and associated error bars directly from raw unique molecular identifier (UMI) counts.
• Parameter-Free Operation: Functions without any tunable parameters.

Scientific Applications:

• Single-Cell Gene Expression Analysis: Normalizes and quantifies gene expression with error estimates from UMI-based single-cell RNA sequencing data.

Methodology:

Bayesian normalization derived from first principles applied directly to raw UMI counts.