seahtrue

seahtrue organizes oxygen consumption and extracellular acidification analysis data from experiments performed on an XF analyzer into structured nested tibbles for detailed processing of raw data, advanced data visualization, and statistics.

Key Features:

• Structured Data Organization: Organizes XF analyzer data into nested tibbles enabling detailed processing of raw data and advanced data visualization and statistics.
• Open and Reproducible Analysis: Introduces an open and reproducible way to analyze XF experiments using file paths to .xlsx files.
• Multi-sheet Data Extraction: Extracts data from four sheets in .xlsx files: Assay Information, Rate Data (OCR and ECAR rates), Raw Data, and Calibration Data.
• Data Preprocessing: Prepares extracted data for calculations through preprocessing steps.
• Data Validation: Performs thorough checks on both initial data and preprocessed data to ensure accuracy.

Scientific Applications:

• Oxygen Consumption Analysis: Processes oxygen consumption rate (OCR) data from XF analyzer experiments for metabolic studies.
• Extracellular Acidification Analysis: Processes extracellular acidification rate (ECAR) data from XF analyzer experiments for metabolic studies.

Methodology:

seahtrue uses file paths to .xlsx files generated by the user in Wave software from Agilent from assay result files (.asyr), extracts specific data needed for analysis from four sheets, prepares the data for calculations through preprocessing, and performs thorough checks on both initial and preprocessed data.