tomoda

tomoda analyzes and visualizes tomo-seq data to identify zones with similar transcriptional profiles and spatially expressed genes in tissue sections.

Key Features:

• Zone detection: Identifies regions with similar transcriptional profiles within tomo-seq samples.
• Spatial gene expression analysis: Detects genes that exhibit spatial expression patterns across consecutive tissue sections.
• Visualization functions: Generates plots that can be modified for tomo-seq data presentation.

Scientific Applications:

• Tomo-seq data analysis: Processes genome-wide expression data with spatial resolution obtained from cryosectioned tissue samples analyzed by RNA-seq on consecutive sections.
• Spatial transcriptomics: Characterizes spatial organization of gene expression in tissues.

Methodology:

The package analyzes data from the tomo-seq technique, which involves cryosectioning of tissue and performing RNA-seq on consecutive sections.