BIOISIS

BIOISIS analyzes small angle X-ray scattering (SAXS) data to characterize the structural dynamics and interactions of biological macromolecules, including proteins and protein-ssDNA complexes such as Replication Protein A (RPA).

Key Features:

• Data Integration: BIOISIS integrates SAXS experimental data with computational simulations to provide a holistic view of macromolecular structures.
• Structural Dynamics Analysis: BIOISIS examines flexibility and orientation changes in protein domains relevant to functional mechanisms.

Scientific Applications:

• RPA structural dynamics: Analysis of Replication Protein A (RPA), including the RPA70 heterotrimeric protein and constructs RPA70AB and RPA70NAB, to investigate domain organization and flexibility.
• Interdomain flexibility: Identification of significant flexibility between the A and B domains of RPA70AB relevant to its function.
• ssDNA binding effects and domain independence: Observation that ssDNA binding reduces flexibility between RPA70AB domains while RPA70N remains structurally independent, supporting its role as a protein recruitment module.

Methodology:

Integration of SAXS experimental data with computational simulations.