BioSyn Lab Calculators
Free browser-based calculators for molecular biology, biochemistry, cell culture and molecular cloning work. Twenty-plus tools cover molarity and dilution, stock and working solutions, DNA and protein molecular weight, primer melting temperature, PCR master mix setup, qPCR efficiency and delta-delta Ct, A260 and A280 concentration, plasmid molecular weight, ligation ratio, transformation efficiency, hemocytometer cell counting, trypan blue viability, seeding density, population doubling time and CFU plate counts, organised into laboratory, DNA & RNA, PCR & qPCR, cell culture, protein and molecular cloning categories. Every result page states the equation, defines each variable and walks a worked example. Each calculator is paired with a method guide explaining the formula, its assumptions and the common mistakes. All computation runs locally in the browser: no input data is uploaded, nothing is stored, and no account is required.
Topics
Details
- License:
- Freeware
- Maturity:
- Emerging
- Cost:
- Free of charge
- Tool Type:
- web application, workflow
- Programming Languages:
- JavaScript
- Added:
- 10/6/2026
- Last Updated:
- 10/6/2026
Operations
Data Inputs & Outputs
Calculation
Inputs
Outputs
Calculation
Conversion
Statistical calculation
Calculation
Expression analysis
Calculation
Calculation
Calculation
Documentation
User manual
https://biosyn-inc.com/guides/how-to-calculate-plasmid-molecular-weight/660 g/mol.bp convention, then pmol/ug, molarity, molecules and copy number.
User manual
https://biosyn-inc.com/guides/how-to-calculate-dna-concentration/A260 to concentration: the 50/33/40 factors, dilution factor, path length, ug/mL to nM.
User manual
https://biosyn-inc.com/guides/c1v1-equation/C1V1 = C2V2 explained: all four rearrangements, the unit-matching rule, and 10x stock mapping.
User manual
https://biosyn-inc.com/guides/how-to-calculate-cfu/CFU/mL from colony counts: dilution factor, plated volume, the 25-250 countable range.
User manual
https://biosyn-inc.com/guides/how-to-calculate-transformation-efficiency/CFU/ug: plated-volume dilution factor, DNA mass to ug, read against the expected range.
General
https://biosyn-inc.com/cell-culture/Cell culture index: counting, viability, seeding density, doubling time, CFU plate counts.
General
https://biosyn-inc.com/dna-rna/DNA and RNA index: GC content, primer Tm, molecular weight, A260 concentration.
User manual
https://biosyn-inc.com/guides/how-to-determine-protein-concentration-a280/Derive epsilon280 from Trp/Tyr/Cys, apply Beer-Lambert, convert to mg/mL and uM.
User manual
https://biosyn-inc.com/guides/how-to-calculate-a-dilution/Find stock volume, diluent volume, dilution factor or final concentration, with ratio and percentage tables.
User manual
https://biosyn-inc.com/guides/how-to-calculate-dna-molecular-weight/Free-acid residue masses, terminal correction, 5'-OH vs 5'-phosphate, ug-to-nmol.
User manual
https://biosyn-inc.com/guides/how-to-calculate-gc-content/GC% = (G + C) / length x 100 with IUPAC degenerate-base rules.
User manual
https://biosyn-inc.com/guides/how-to-calculate-ligation-ratio/Insert:vector molar ratio from fragment sizes and vector mass, or reversed from masses used.
User manual
https://biosyn-inc.com/guides/how-to-calculate-protein-isoelectric-point/Ionizable groups, pKa values, the net-charge sum and why iteration is required.
General
https://biosyn-inc.com/laboratory/Laboratory calculator index: molarity, dilution, stock solutions, percent and ppm.
User manual
https://biosyn-inc.com/guides/how-to-calculate-delta-delta-ct/Livak 2^-ddCt by hand: normalise to a reference gene, subtract control, check efficiency first.
User manual
https://biosyn-inc.com/guides/how-to-make-a-stock-solution/Mass = MW x M x V, the 80% rule, hydrate and purity corrections, per-compound recipes.
User manual
https://biosyn-inc.com/guides/how-to-calculate-molarity/Molarity in three steps with worked examples for NaCl, glucose and a 10 mM reagent.
General
https://biosyn-inc.com/cloning/Molecular cloning index: plasmid MW, ligation ratio, transformation efficiency.
User manual
https://biosyn-inc.com/guides/molarity-vs-molality/Moles per litre vs per kg of solvent, the temperature difference, and M vs m vs N.
General
https://biosyn-inc.com/pcr/PCR and qPCR index: primer Tm, master mix setup, qPCR standard-curve efficiency.
User manual
https://biosyn-inc.com/guides/how-to-make-a-serial-dilution/Plan a serial dilution: tube-by-tube concentrations, transfer and diluent volumes, pipetting errors.
General
https://biosyn-inc.com/protein/Protein index: molecular weight, isoelectric point, A280 concentration.
User manual
https://biosyn-inc.com/guides/how-to-calculate-qpcr-efficiency/Regress Cq on log10 concentration, why slope -3.32 is 100%, the 90-110% window.
User manual
https://biosyn-inc.com/guides/how-to-make-a-pcr-master-mix/Solve each component with C1V1=C2V2, scale by reactions plus overage, balance the water.
User manual
https://biosyn-inc.com/guides/how-to-calculate-molar-mass/Sum atomic masses, handle hydrates, convert grams to moles; H2O, NaCl and glucose examples.
User manual
https://biosyn-inc.com/guides/how-to-calculate-protein-molecular-weight/Sum residue masses and add one water; average vs monoisotopic; sources.
User manual
https://biosyn-inc.com/guides/how-to-calculate-seeding-density/Target density to cells per well, growth-area conversion, C1V1=C2V2 for the stock volume.
User manual
https://biosyn-inc.com/guides/how-to-calculate-doubling-time/Td = t.ln2/ln(Nt/N0), the log2 form, specific growth rate, PDL vs passage number.
User manual
https://biosyn-inc.com/guides/how-to-count-cells-with-hemocytometer/The 1e-4 mL chamber volume, why you multiply by 10^4, the border-cell rule.
User manual
https://biosyn-inc.com/guides/percent-solution-wv-vv-ww/The four percent-solution types compared, plus converting w/v to w/w using density.
User manual
https://biosyn-inc.com/guides/how-to-choose-annealing-temperature/Tm-5 empirical start, Rychlik Ta-optimum formula, gradient windows, mismatched primers.
User manual
https://biosyn-inc.com/guides/how-to-calculate-cell-viability/Trypan blue counts: viability % = live/total x 100, viable density, the ~90% threshold.
User manual
https://biosyn-inc.com/guides/how-to-prepare-a-working-solution/Turn a stock into a working solution: dilution-factor table, workflow, ratio crosswalk.
User manual
https://biosyn-inc.com/guides/how-to-calculate-primer-tm/Wallace rule and SantaLucia 1998 nearest-neighbour method with a worked example.
User manual
https://biosyn-inc.com/guides/what-is-molarity/What molarity means: moles per litre, M vs mM, and why temperature changes the answer.
User manual
https://biosyn-inc.com/guides/ppm-to-mg-l/Why 1 ppm is about 1 mg/L only in dilute water; crosswalk to ug/mL, ppb, % and mg/m3.