BIPS

BIPS converts protein lists into custom DNA oligonucleotide libraries for PhIP-Seq to enable high-throughput immunoprecipitation sequencing and antibody repertoire mapping.

Key Features:

• Custom Library Creation: Generates DNA oligonucleotide libraries directly from input protein lists for targeted PhIP-Seq experiments.
• Constant-Length Oligonucleotides with Internal Barcodes: Produces constant-length oligonucleotides embedded with internal barcodes for sequence tracking and identification.
• Peptide Length Preservation: Standardizes oligonucleotide length while preserving original peptide lengths to retain biological relevance.
• Scalability and Cost Efficiency: Supports libraries of hundreds of thousands of oligonucleotides, reducing sequencing costs while maintaining high accuracy in read identification.

Scientific Applications:

• PhIP-Seq and immunoprecipitation sequencing: Creation of large, customized oligonucleotide libraries to identify protein-DNA interactions and map antibody repertoires.

Methodology:

Converts input protein lists into DNA sequences compatible with the PhIP-Seq system, generates constant-length oligonucleotides that preserve original peptide lengths, and tags each sequence with an internal barcode for tracking.