BiQ Analyzer HT

BiQ Analyzer HT analyzes locus-specific high-throughput bisulfite sequencing data to quantify and visualize DNA methylation patterns in eukaryotic genomes.

Key Features:

• Locus-Specific Analysis: Focuses analysis on specific genomic loci using bisulfite-converted DNA, applicable to experiments employing locus-specific PCR primers or DNA capture probes.
• Visualization Capabilities: Provides visualization of methylation patterns at selected loci and supports integration of genome-wide bisulfite sequencing data with locus-focused inspection.
• Quantitative Accuracy: Delivers single-base-pair resolution methylation assessment with quantitative accuracy contingent on sequencing depth.
• Support for Targeted and Genome-wide Data: Handles both targeted (locus-specific) and genome-wide bisulfite sequencing datasets.
• High-throughput Alternative to Clonal Methods: Enables analysis of high-throughput bisulfite sequencing data as an alternative to traditional clonal bisulfite sequencing approaches.

Scientific Applications:

• DNA methylation profiling: Enables precise quantification and visualization of locus-specific DNA methylation patterns for epigenetic studies.
• Gene regulation and development studies: Supports investigations into methylation-mediated regulation of gene expression and developmental processes in eukaryotes.
• Disease and pathogenesis research: Facilitates analysis of methylation changes relevant to cancer and neurological disorders and other disease-related epigenetic alterations.
• Targeted epigenetic investigations: Applicable to studies using locus-specific PCR primers or DNA capture probes to interrogate defined genomic regions.

Methodology:

Processes high-throughput bisulfite sequencing data to quantify and visualize DNA methylation at specific genomic loci, supporting both targeted and genome-wide datasets.