BISCAP

BISCAP processes 2D Optical Coherence Tomography (OCT) biofilm images to automatically extract structural parameters and quantify biofilm architecture.

Key Features:

• Automatic Processing: Automates processing of 2D OCT biofilm images to replace custom-script workflows.
• Novel Algorithmic Approaches: Incorporates principles from Confocal Laser Scanning Microscopy (CLSM) image processing and implements a unique thresholding algorithm and substratum detection strategy.
• Detailed Pixel Characterization: Employs two complementary pixel continuity checks for precise per-pixel characterization within biofilm images.
• Comprehensive Output: Produces common structural parameters used in biofilm analysis and a set of processed images for examination and comparison.
• Innovative Biofilm Compaction Parameter: Introduces a novel parameter to quantify biofilm compaction.

Scientific Applications:

• Biofilm structural analysis: Quantifies biofilm architecture and structural parameters from 2D OCT imagery, including compaction metrics.
• Comparative studies: Provides consistent structural metrics to support comparative analyses across different datasets or experimental conditions.

Methodology:

Applies advanced image processing techniques that incorporate CLSM image processing principles, a unique thresholding algorithm, a substratum detection strategy, and two complementary pixel continuity checks.