Bismark

Bismark maps bisulfite-treated high-throughput sequencing reads and performs methylation calling to profile genome-wide cytosine methylation at single-base resolution.

Key Features:

• Integrated mapping and calling: Integrates read mapping and methylation calling into a single processing step.
• Bisulfite sequencing support: Processes BS-Seq input from bisulfite-treated high-throughput sequencing reads.
• Single-base resolution: Reports methylation calls at single-base resolution across the genome.
• Sequence-context discrimination: Distinguishes cytosines in CpG, CHG, and CHH sequence contexts.
• Genome-wide profiling: Enables analysis of genome-wide cytosine methylation patterns.

Scientific Applications:

• BS-Seq data analysis: Analysis of BS-Seq (bisulfite sequencing) data to derive cytosine methylation calls.
• Epigenomic profiling: Genome-wide cytosine methylation profiling for epigenomics studies.
• Single-base methylation studies: Examination of methylation patterns at single-base resolution across genomic regions.

Methodology:

Performs integrated read mapping of bisulfite-treated sequencing reads and methylation calling, reporting context-specific (CpG, CHG, CHH) methylation at single-base resolution.