Blast2GO

Blast2GO performs Gene Ontology (GO)-based functional annotation and analysis of DNA and protein sequence datasets to characterize novel or unannotated transcriptomic and genomic sequences.

Key Features:

• Gene Ontology Annotation: Performs GO annotation via similarity searches and homology transfer to assign GO terms to novel sequences.
• Integrated Analysis Workflow: Provides an end-to-end computational workflow for functional annotation of transcriptomic datasets that combines multiple annotation strategies and tools and allows control over annotation type and intensity.
• Graphical Visualization: Generates GO-graph visualizations and descriptive charts, including directed acyclic graph representations for gene-set function profiling.
• Sequence Management: Manages large volumes of DNA and protein sequences for batch processing and annotation.
• High-Throughput Capabilities: Processes extensive datasets in high-throughput mode to produce scalable annotation results for model and non-model species.

Scientific Applications:

• Functional Genomics: Enables characterization of gene product functions in functional genomics studies through GO annotation and gene-set profiling.
• Annotation of Non-model Species: Facilitates annotation of transcriptomic and genomic sequences from non-model species by transferring annotations via sequence similarity.

Methodology:

Methodology uses similarity searches and homology-based GO term transfer combined with statistical analyses, GO-graph visualization, and annotation strategies that control annotation type and intensity.