BoardION

BoardION monitors and analyzes real-time Oxford Nanopore Technologies (ONT) sequencing runs from MinION, Mk1C, GridION, and PromethION to provide metrics for assessing sequencing quality and optimizing library preparation and experimental conditions.

Key Features:

• Real-Time Monitoring: Streams and analyzes ONT sequencing output in real time to produce immediate run metrics.
• Sequencing Metrics Generation: Processes streamed ONT data to compute comprehensive metrics for sequencing quality and yield.
• Flowcell Comparison: Compares metrics across multiple flowcells to evaluate library preparation protocols and assess input sample quality.
• Data-Driven Optimization: Provides real-time feedback that enables adjustment of sequencing parameters to optimize data quantity and quality.
• ONT Platform Support: Operates with MinION, Mk1C, GridION, and PromethION sequencing platforms.

Scientific Applications:

• Real-Time Run Management: Enables monitoring and on-the-fly adjustments during sequencing experiments to maintain data quality.
• Library Preparation Evaluation: Facilitates comparative assessment of library preparation protocols via flowcell metric comparison.
• Input Sample Quality Control: Assists evaluation of sample quality through flowcell-level metrics.
• Metagenomics and Large-Scale Genomics: Supports projects where immediate assessment of sequencing data quality influences experimental decisions and outcomes.

Methodology:

Integrates with ONT devices to stream real-time data, processes the streamed data to compute comprehensive sequencing metrics, and compares metrics across multiple flowcells.