BonZeb

BonZeb provides modular Bonsai-based processing for high-resolution tracking and analysis of larval zebrafish behavior to enable real-time closed-loop experiments and integration with optogenetic stimulation and calcium imaging.

Key Features:

• High-Resolution Tracking: Performs precise behavioral tracking of larval zebrafish with support for online (real-time) and offline processing and dynamic visual feedback.
• Versatile Experimental Configurations: Supports head-fixed closed-loop assays and free-swimming virtual open-loop assays.
• Multi-Animal Tracking: Enables simultaneous tracking of multiple animals for analyses of interactions and group dynamics.
• Integration with Optogenetics and Calcium Imaging: Interfaces behavioral tracking with optogenetic stimulation and calcium imaging to correlate neural activity with behavior.
• Modular Bonsai Packages: Implemented as modular packages within the Bonsai software platform to enable construction of custom workflows for real-time and offline processing.

Scientific Applications:

• Neural circuit analysis: Dissects neural circuits that underlie larval zebrafish behavior.
• Closed-loop optogenetic experiments: Enables real-time behavioral feedback combined with optogenetic stimulation to correlate neural activity with behavior.
• Correlated imaging-behavior studies: Combines behavioral tracking with calcium imaging to correlate neural activity patterns with high-resolution behavioral metrics.

Methodology:

Implemented as modular packages within the Bonsai software platform, providing real-time (online) behavioral tracking with dynamic visual feedback and offline analysis capabilities.