Breeze

Breeze analyzes high-throughput screening (HTS) data from multi-well plates to perform quality control, dose-response fitting, and calculation of quantitative pharmacological scores for compound evaluation.

Key Features:

• Quality Control: Implements comprehensive quality control procedures to mitigate technical and experimental errors associated with HTS on multi-well plates.
• Dose-Response Fitting and Quantitative Scoring: Performs dose-response curve fitting and computes EC50, IC50, drug sensitivity score (DSS), and area under the curve (AUC).
• Interactive Visualizations: Generates visualizations at each analysis step to support exploration and interpretation of HTS results.
• Functional Precision Medicine Applications: Identifies drug sensitivity and resistance patterns in cell lines and patient-derived samples to support individualized response assessment.

Scientific Applications:

• Drug Discovery and Development: Evaluates compound efficacy and ranks investigational and therapeutic agents from HTS datasets.
• Functional Precision Medicine: Supports assessment of individual cellular responses to inform personalized therapeutic strategies using cell lines or patient-derived samples.

Methodology:

Integrates quality control with data analysis through initial data assessment to filter unreliable results, dose-response curve fitting, calculation of pharmacological parameters (EC50, IC50, DSS, AUC), and generation of visualizations.