breseq_bam2cov

breseq_bam2cov generates coverage plots or tables for specified genomic regions from next-generation DNA sequencing (NGS) data to support analysis of whole-genome and whole-population datasets from haploid microbes using a high-quality reference genome within the Breseq computational pipeline.

Key Features:

• Coverage quantification: Produces per-region coverage plots or tables from NGS data for specified genomic intervals.
• Genetic analysis: Enables identification and annotation of genetic differences within microbial populations.
• Mutation detection: Detects unintended mutations arising during strain construction or genome editing.
• Integration with Breseq: Functions as a component of the Breseq computational pipeline and supports analysis of mutants isolated through genetic screens.

Scientific Applications:

• Eco-evolutionary studies: Reconstructs population dynamics and identifies adaptive genetic changes in laboratory evolution experiments.
• Genome editing validation: Identifies unintended mutations to validate outcomes of strain construction and genome editing experiments.

Methodology:

Generates coverage plots or tables from NGS data as part of the Breseq computational pipeline for analysis of whole-genome and whole-population datasets from haploid microbes using a high-quality reference genome.