BS-virus-finder

BS-virus-finder detects viral integration breakpoints within whole human genomes using bisulfite sequencing data to link integration loci with DNA methylation patterns that can affect gene expression and carcinogenesis.

Key Features:

• Detection of viral integration breakpoints: Identifies viral integration sites within host genomic sequences using bisulfite sequencing data.
• Analysis of DNA methylation: Analyzes DNA methylation patterns at integration loci to relate integrations to epigenetic changes.
• Epigenetic research applicability: Enables investigation of the relationship between viral integration, DNA methylation, and gene regulation.
• High sensitivity and specificity: Demonstrates high sensitivity and specificity for detecting viral integration loci as reported.
• Novel application of bisulfite sequencing: Applies bisulfite sequencing data specifically to detect viral integration loci, extending bisulfite sequencing use beyond single nucleotide polymorphism calling and differentially methylated region identification.

Scientific Applications:

• Epigenetic studies: Investigation of how viral integration influences DNA methylation patterns and gene expression.
• Virus-induced carcinogenesis research: Studies of molecular mechanisms by which viral integrations may contribute to carcinogenesis.
• Genome-wide integration mapping: Mapping and characterization of viral integration loci across whole human genomes using bisulfite sequencing data.

Methodology:

Processes bisulfite sequencing data to detect regions where viral DNA has integrated into the host genome and analyzes methylation changes at those sites to pinpoint integration breakpoints.