BSRD

BSRD provides a comprehensive repository and analysis resources for bacterial small regulatory non-coding RNAs (sRNAs) to support identification, annotation, and functional study of sRNA-mediated post-transcriptional regulation.

Key Features:

• Extensive Repository: Hosts experimentally validated bacterial sRNA sequences with detailed annotations and expression profiles.
• Regulatory Network Integration: Contains combinatorial regulatory networks mapping interactions between transcription factors, sRNAs, and their common targets.
• High-Throughput Data Analysis (sRNADeep): Incorporates the sRNADeep RNA-Seq analysis platform for detection and characterization of sRNAs from genomic tiling arrays and RNA-Seq data.

Scientific Applications:

• Gene Regulation Studies: Enables investigation of sRNA roles in bacterial post-transcriptional gene regulation, including quorum sensing, stress response, virulence, and carbon metabolism.
• Pathogenesis and Virulence Research: Supports analysis of sRNA-mediated regulatory mechanisms implicated in bacterial virulence.
• Transcriptome-scale sRNA Discovery: Facilitates discovery and characterization of sRNAs from high-throughput transcriptome datasets such as RNA-Seq and genomic tiling arrays.
• Regulatory Network Reconstruction: Aids reconstruction and analysis of combinatorial regulatory networks involving transcription factors, sRNAs, and target genes.

Methodology:

Incorporation of the sRNADeep RNA-Seq analysis platform; detection and characterization of sRNAs using genomic tiling arrays and RNA-Seq; integration and mapping of combinatorial regulatory networks linking transcription factors, sRNAs, and targets.