BugBuster

BugBuster streamlines metagenomic data analysis through a modular, containerized workflow implemented in Nextflow that processes reads, contigs, and metagenome-assembled genomes for taxonomic profiling and resistome characterization.

Key Features:

• Modular Architecture: The workflow consists of dedicated modules for taxonomic profiling and resistome characterization.
• Containerization: BugBuster uses containers for deployment with minimal configuration on workstations, high-performance clusters, or cloud platforms.
• Multi-level Analysis: The pipeline analyzes data at the level of reads, contigs, and metagenome-assembled genomes.

Scientific Applications:

• Taxonomic Profiling: Characterization of microbial community composition from metagenomic sequencing data.
• Resistome Characterization: Identification and analysis of antimicrobial resistance genes within microbial communities.
• Metagenomic Dataset Analysis: Robust, scalable, and reproducible processing of massive metagenomic datasets capturing the complete genetic content of samples.

Methodology:

BugBuster was written in Nextflow-DSL2.