Buttery-eel

Buttery-eel accelerates nanopore basecalling by enabling Oxford Nanopore's Guppy to read SLOW5-formatted raw current signal data for efficient conversion into DNA and RNA sequence reads.

Key Features:

• Integration with Guppy Basecaller: Acts as an open-source wrapper for Oxford Nanopore's Guppy basecaller to enable processing of SLOW5 files.
• Utilization of SLOW5 Format: Leverages the SLOW5 file format to permit efficient sequential access to raw current signal data.
• Performance Improvements: Reduces data-access bottlenecks in HPC and cloud environments to improve throughput and scalability of nanopore basecalling.

Scientific Applications:

• High-throughput nanopore sequencing: Facilitates large-scale basecalling of DNA and RNA datasets generated by nanopore sequencing.
• Metagenomics: Supports scalable processing of mixed-community nanopore sequencing data for metagenomic analyses.
• Transcriptomics: Improves basecalling throughput for nanopore RNA sequencing to support transcriptomic studies.

Methodology:

Integrates SLOW5 sequential data-access with Guppy basecalling to process raw nanopore current signal data into sequence reads and is implemented to operate in HPC and cloud environments.