CalibraCurve

CalibraCurve enables automated batch-mode determination of dynamic linear ranges and quantification limits for targeted proteomics and similar assays using SRM/MRM-MS-based measurements.

Key Features:

• Automated batch-mode calibration: Determines dynamic linear ranges and quantification limits for multiple analytes simultaneously.
• Accuracy assessment: Uses precision and trueness measures to evaluate calibration quality.
• Customizable visualizations: Generates calibration curves and response factor plots to assess model fit.

Scientific Applications:

• Targeted proteomics assay development: Facilitates development and control of reliable SRM/MRM-MS-based proteomics assays with quantitative measurements spanning 4-5 orders of magnitude in complex matrices.
• Protein assay validation: Supports robust protein assay development for sensitive applications such as health care by determining reliable quantification limits.

Methodology:

The software performs automated batch-mode determination of dynamic linear ranges and quantification limits, assesses calibration accuracy through precision and trueness measures, and generates customizable calibration curves and response factor plots.