CarbArrayART

CarbArrayART manages storage, processing, presentation, and reporting of glycan microarray data to support identification of glycan ligands and reproducible glycomics experiments.

Key Features:

• Data storage and management: Stores quantified array data from diverse array layouts along with associated metadata.
• Scan data and metadata capture: Incorporates imaging scan data and array-specific metadata including lists of arrayed glycans, array geometry, glycan-binding sample details, and experimental protocols.
• Quantification: Calculates average fluorescence intensity values using imaging scan data and array geometry.
• Data presentation: Generates charts, tables, and heatmaps derived from averaged fluorescence intensity values.
• Filtering and sorting: Enables filtering and sorting of results based on monosaccharide content and glycan sequences.
• Export and reporting: Exports data and reports in Word, PDF, and Excel formats and includes metadata compliant with MIRAGE guidelines.

Scientific Applications:

• Slide-based glycan microarray experiments: Streamlines routine tasks for design, storage, processing, and reporting of slide-based glycan microarray studies.
• Glycan ligand identification: Supports analysis workflows used to identify glycan ligands across glycan recognition systems.
• Reproducible glycomics and data transparency: Facilitates MIRAGE-compliant reporting to improve data transparency, reproducibility, and validity in glycobiology research.

Methodology:

Computes average fluorescence intensity values from imaging scan data using array geometry, generates charts/tables/heatmaps from those values, supports filtering and sorting by monosaccharide content and glycan sequences, stores quantified array data and metadata, and exports MIRAGE-compliant reports in Word, PDF, and Excel formats.