CBP60-DB

CBP60-DB provides predicted protein structures and bioinformatic annotations for the CALMODULIN-BINDING PROTEIN 60 (CBP60) family across the plant kingdom to support comparative analysis of sequence, structure, domain architecture, and functional conservation.

Key Features:

• Extensive Coverage: Characterizes 1052 CBP60 gene homologs encoding 2376 unique transcripts and 1996 unique proteins across 62 phylogenetically diverse plant genomes.
• Structural Prediction: Predicts three-dimensional structures for CBP60 proteins using AlphaFold2 (deep learning).
• Novel Clustering Visualization Algorithm: Applies a novel clustering visualization algorithm to interrogate kingdom-wide structural similarities among CBP60 proteins.
• Integration with External Bioinformatics Resources: Integrates external resources to analyze protein domains and motifs, with emphasis on calmodulin-binding domains of CBP60 proteins in Arabidopsis thaliana.

Scientific Applications:

• Growth and Stress Signaling: Supports analysis of CBP60 roles in plant growth and stress-signaling pathways.
• Plant Immune Response Studies: Enables investigation of CBP60 involvement with cell surface receptors, MAP kinases, WRKY transcription factors, and biosynthetic enzymes for salicylic acid (SA) and N-hydroxypipecolic acid (NHP).
• Comparative and Evolutionary Analysis: Facilitates cross-species comparison of CBP60 sequence and predicted structure to infer functional conservation across diverse plant taxa.

Methodology:

Structural models were generated with AlphaFold2 (deep learning); a novel clustering visualization algorithm assessed kingdom-wide structural similarities; and external bioinformatics resources were integrated to analyze protein domains and motifs, including calmodulin-binding domains.