CentromereArchitect

CentromereArchitect annotates and decomposes centromeric assemblies to identify human monomers and high-order repeats (HORs) for analysis of centromere architecture and evolution.

Key Features:

• Centromere annotation: Annotates newly assembled centromeric regions to identify complete sets of human monomers and high-order repeats (HORs) with explicit algorithmic definitions.
• Support for long-read assemblies: Processes assemblies derived from long-read sequencing technologies, including assemblies generated by the Telomere-to-Telomere (T2T) consortium, to enable comprehensive centromere annotation.
• Hybrid monomer discovery: Detects and reports hybrid monomers observed in complete human genome assemblies.
• Decomposition algorithms: Decomposes complex centromeric assemblies into constituent monomers and HORs using specialized bioinformatics algorithms.
• Enables comparative and population analyses: Produces annotations suitable for evolutionary studies and population- or individual-level investigations of centromere variation.

Scientific Applications:

• Human centromere architecture: Characterize the organization of monomers and HORs in human centromeric regions.
• Centromere evolution: Identify hybrid monomers and other features that inform studies of centromere evolutionary dynamics.
• Population and biomedical studies: Compare centromeric variation across individuals to support genotype–phenotype and personalized biomedical research.
• Comparative genomics: Compare centromeric architectures across species to investigate evolutionary conservation and divergence.

Methodology:

Applies specialized bioinformatics algorithms to decompose centromeric assemblies into constituent monomers and high-order repeats (HORs).