CFM

CFM catalogs experimentally validated protocols for chemical compound-based direct reprogramming and transdifferentiation of cells to enable selection and comparison of high-yield cell conversion methodologies.

Key Features:

• Standardized protocol representation: Protocols are preprocessed, verified, and represented in a standardized format to ensure consistency across entries.
• Content scope: The database encompasses 169 distinct protocols enabling 113 types of cell conversions using 158 small molecules.
• Chemical annotation: Each protocol entry includes PubChem Compound IDs (CIDs) and Mechanisms Of Action (MOA) for involved chemicals.
• Protocol metadata: Entries provide duration, media used, yield metrics, and associated measurement strategies for each protocol.
• Experimentally validated protocols: All protocols in the collection are reported as experimentally validated for the specified conversions.

Scientific Applications:

• Stem cell biology: Facilitating selection and replication of chemical reprogramming protocols in stem cell research.
• Cell fate engineering: Informing development and optimization of direct reprogramming and transdifferentiation strategies.
• Regenerative medicine: Supporting generation of target cell types for regenerative medicine applications.
• Disease modeling: Enabling production of specific cell types for in vitro disease models.
• Therapeutic development: Aiding identification and comparative assessment of protocols for therapeutic cell production.

Methodology:

Protocols are preprocessed, verified, and represented in a standardized format; entries include PubChem CIDs and recorded Mechanisms Of Action, duration, media, yield metrics, and measurement strategies.