ChanFAD

ChanFAD provides integrated functional annotations of ion channels by linking primary sequence information, two-dimensional interactions, and three-dimensional PDB structures for structural and functional analysis using NCBI's iCn3D.

Key Features:

• Functional Annotation: Links ion channel primary sequence data to two-dimensional interaction annotations and three-dimensional protein structures.
• Structure Visualization: Utilizes NCBI's iCn3D to view and explore PDB structures in the context of annotated functional regions.
• Comprehensive Data Integration: Associates organism-specific variations and cross-references to other databases alongside sequence and structural annotations.

Scientific Applications:

• Drug–channel interaction analysis: Supports analysis of how ligands and drugs interact with annotated ion channel regions on PDB structures.
• Mutation structural effect analysis: Enables examination of structural changes induced by mutations mapped onto three-dimensional channel models.
• Comparative and organism-specific studies: Facilitates comparison of sequence and structural variation across organisms using linked annotations.

Methodology:

Maps primary sequence annotations and two-dimensional interactions to PDB structures and displays them using NCBI's iCn3D structure viewer.