ChromatoShiny

ChromatoShiny provides interactive analysis and visualization of chromatography profiles to enhance annotation, customization, and export of Unicorn™ outputs from Äkta liquid chromatography instruments.

Key Features:

• Unicorn™/Äkta input support: Accepts and processes chromatography outputs generated by Unicorn™ on Äkta liquid chromatography instruments.
• Interactive visualization: Produces dynamic chromatogram plots for exploratory inspection and selection of regions of interest.
• Baseline fitting: Fits baselines to chromatographic traces to support accurate quantification of peak areas and peak heights.
• Annotation of fractions and elution volumes: Adds annotations for specific fractions and elution volumes directly onto chromatograms.
• Highlighting of regions: Emphasizes selected areas within or beneath chromatogram plots to mark peaks or fractions.
• Zoom functionality: Enables focused examination of specific chromatogram segments at higher resolution.
• Customization and export: Provides options to customize annotated plots and export annotated figures and associated data.

Scientific Applications:

• Macromolecule purification and analysis: Supports analysis of chromatograms from purification workflows for biological macromolecules used in biochemistry and molecular biology.
• Downstream biochemical assays: Facilitates selection and documentation of fractions for biochemical assays.
• Biophysical characterization: Aids identification of fractions and peaks for biophysical measurements.
• Structural studies: Documents and annotates fractions intended for structural biology workflows.
• Figure generation: Produces annotated chromatograms suitable for inclusion in publications and reports.

Methodology:

ChromatoShiny is implemented in R using the Shiny framework for interactive chromatogram visualization and annotation.