Circadian Ontogenetic Metabolomics Atlas (COMA)

Circadian Ontogenetic Metabolomics Atlas (COMA) maps circadian metabolic rhythms across 16 rat anatomical structures during development using multiplatform liquid chromatography-mass spectrometry data.

Key Features:

• Multi-tissue coverage: Integrates metabolomics data from brain regions (suprachiasmatic nuclei, medial prefrontal cortex) and peripheral tissues (liver, plasma) plus fecal samples across developmental stages.
• Developmental time course: Spans embryonic E19 through postnatal stages P2, P10, P20, and P28, with brain and liver/plasma tissues sampled at all stages and additional peripheral tissues at P20 and P28.
• Metabolite annotation: Annotates 851 metabolites from 1610 samples using liquid chromatography-mass spectrometry.
• Circadian analysis: Identifies circadian oscillations using JTK_CYCLE, detecting an average of 24% of metabolites exhibiting circadian rhythms across sample types.
• Stage transition analysis: Detects distinct circadian shifts during the transition from nursing to solid food intake (P10-P20).

Scientific Applications:

• Circadian metabolic regulation: Explores how circadian rhythms regulate metabolism across central and peripheral tissues during development.
• Developmental biology: Investigates temporal dynamics of metabolism across tissues when circadian gene expression has been well studied but metabolic dynamics remain less understood.
• Sampling standardization: Informs standardized metabolomics sampling protocols by demonstrating that metabolite intensities fluctuate with both circadian rhythms and development.

Methodology:

Multiplatform liquid chromatography-mass spectrometry analysis of metabolites; circadian oscillation detection by JTK_CYCLE.