circAtlas

circAtlas catalogs circular RNAs (circRNAs) from multi-species RNA-seq data to support transcriptomic, comparative, and functional analyses.

Key Features:

• Extensive Database: Contains 1,007,087 circRNAs with 81.3% assembled into full-length sequences.
• Multi-species RNA-seq Integration: Built from the integration of 1,070 RNA-seq samples collected across 19 normal tissues from six vertebrate species.
• High Reliability: Emphasizes accuracy of entries through assembly of circRNA sequences into full-length forms.
• Expression Profiling: Provides expression profiles of circRNAs across different tissues and species.
• Conservation Analysis: Includes a multiple conservation score to assess evolutionary preservation of circRNAs.
• Functional Annotation: Supplies functional annotations to infer potential biological roles and interactions of circRNAs.
• Co-expression Networks: Profiles co-expression patterns to reveal potential regulatory relationships between circRNAs and other genomic elements.
• Regulatory Networks: Provides information on regulatory networks involving circRNAs and their putative regulatory partners.

Scientific Applications:

• Candidate Prioritization: Supports prioritization of circRNA candidates for experimental validation.
• Transcriptomics Research: Enables investigation of circRNA expression patterns and potential functions across tissues and species.
• Functional Genomics: Facilitates hypothesis generation about circRNA roles using functional annotations and network data.
• Comparative Studies: Allows cross-species comparison of circRNA conservation and divergence among vertebrates.
• Biomarker Discovery: Supports identification of candidate circRNA biomarkers using expression and co-expression data.

Methodology:

Integrated 1,070 RNA-seq samples from 19 normal tissues across six vertebrate species; assembled circRNA sequences into full-length forms (81.3% full-length); computed a multiple conservation score; profiled expression across tissues and species; generated co-expression and regulatory network data; and produced functional annotations.