Citrusgreening.org

Citrusgreening.org provides an integrated systems biology resource for studying the Huanglongbing (HLB) disease complex across host Citrus species, the Asian citrus psyllid (ACP), and pathogens including Candidatus Liberibacter asiaticus.

Key Features:

• Holistic Pathosystem Framework: Implements a pathosystem-wide data model integrating host Citrus, Asian citrus psyllid (ACP), and Liberibacter pathogen data.
• Data Submission and Sharing: Supports submission and centralized sharing of community datasets for integrated analysis.
• Omics Data Tools (CitrusCyc, DiaphorinaCyc): Provides metabolic pathway databases CitrusCyc and DiaphorinaCyc for mining metabolomics, transcriptomics, and proteomics data.
• Expression Networks (PEN, CEN): Hosts the Psyllid Expression Network (PEN) and Citrus Expression Network (CEN) with gene expression profiles across life stages, tissues, conditions, and hosts.
• Integrated Tools (Apollo/JBrowse, BLAST, BioCyc): Connects Apollo/JBrowse genome browsers, BLAST, and BioCyc tools to a central gene model database for Citrus, ACP, and Liberibacter pathogens.
• Additional Experimental Data: Includes electrical penetration graph (EPG) recordings of ACP feeding, citrus rootstock trial data, and metabolomics datasets.
• Manual Curation Tools: Provides manual curation functionality to refine gene models and annotations.

Scientific Applications:

• Disease Mechanism Analysis: Enables integrated analysis of host, vector, and pathogen omics to investigate Huanglongbing (HLB) disease mechanisms.
• Host–Pathogen–Vector Interaction Studies: Supports analysis of interactions among Citrus species, ACP, and Candidatus Liberibacter asiaticus using genome, expression, and pathway data.
• Pathway and Network Discovery: Facilitates mining of metabolic pathways and regulatory networks using CitrusCyc, DiaphorinaCyc, PEN, and CEN.
• Feeding Behavior and Agronomic Studies: Allows incorporation of EPG recordings, rootstock trial data, and metabolomics for vector behavior and host response investigations.

Methodology:

Integrates Apollo/JBrowse, BLAST, BioCyc (CitrusCyc, DiaphorinaCyc), expression networks (PEN, CEN), and manual curation against a central gene model database for Citrus, Asian citrus psyllid (ACP), and Candidatus Liberibacter asiaticus.