clampFISH

clampFISH 2.0 enhances in situ RNA labeling by using an inverted padlock design and multiplexed exponential signal amplification to visualize and quantify transcript levels in single cells and tissue sections.

Key Features:

• Inverted padlock design: Employs an inverted padlock probe architecture to enable specific probe binding and signal amplification.
• Exponential signal amplification: Amplifies target RNA signals exponentially to increase detection sensitivity.
• Multiplexed detection: Enables simultaneous identification of multiple RNA species within the same sample.
• Detection capacity: Supports simultaneous identification of up to ten different RNA species.
• High throughput scalability: Demonstrated scalability to profile RNA across over one million cells.
• Sequential detection: Uses sequential detection processes to expand multiplexing and signal readout.
• Applicability to tissue sections: Compatible with tissue section samples in addition to single cells.
• Reduced time and cost: Reportedly reduces both assay time and reagent costs relative to the previous version.
• Visualization and quantification: Facilitates visualization and quantification of transcript levels for spatial analysis.

Scientific Applications:

• Spatial transcriptomics: Maps RNA distribution and expression patterns within spatial contexts of tissues and cells.
• Single-cell gene expression analysis: Measures transcript levels at single-cell resolution for studies of cellular heterogeneity.
• Tissue-level transcript profiling: Enables analysis of gene expression dynamics across tissue sections.

Methodology:

Uses an inverted padlock probe design with multiplexed signal amplification combined with sequential detection processes.