click-ExM

click-ExM enables nanoscale visualization of diverse biomolecules by integrating click chemistry labeling with Expansion Microscopy (ExM) to allow imaging on conventional fluorescence microscopes.

Key Features:

• Integration of Click Chemistry: Labels biomolecules via click chemistry with biotin followed by staining with fluorescently labeled streptavidin for fluorescence detection.
• Versatility in Biomolecule Imaging: Supports 18 different clickable labels for imaging lipids, glycans, proteins, DNA, RNA, and small molecules.
• Compatibility with Standard ExM Procedures: Operates with the standard ExM procedure typically used for protein imaging.
• Applicability in Various Systems: Applicable to cell culture and tissue imaging.
• Signal-Amplification and Multi-Color Imaging: Maintains compatibility with signal-amplification techniques and supports two-color imaging.

Scientific Applications:

• Cellular and Molecular Imaging: Nanoscale visualization of lipids, glycans, proteins, DNA, RNA, and small molecules within cells to study subcellular architecture.
• Tissue Analysis: Nanoscale imaging of biomolecules in tissue samples for spatial and structural studies.

Methodology:

Biomolecules are labeled by click chemistry with biotin, stained with fluorescently labeled streptavidin, and subjected to standard Expansion Microscopy (ExM) procedures.