Click-qPCR

Click-qPCR streamlines ΔCq and ΔΔCq calculations, statistical testing, and visualization for qPCR gene expression and DNA copy number variation analysis.

Key Features:

• ΔCq and ΔΔCq calculations: Performs relative quantification calculations from user-uploaded CSV data files.
• Statistical testing: Implements Welch's t-tests for pairwise comparisons and one-way analysis of variance with Dunnett's post-hoc test for multi-group comparisons.
• Interactive visualization: Generates interactive bar plots displaying mean ± standard deviation with individual data points.
• Results export: Produces publication-quality images and summary statistics for download.
• Group and gene selection: Enables interactive selection of genes, experimental groups, and reference genes for analysis.

Scientific Applications:

• Gene expression analysis: Quantifies relative gene expression levels across various conditions and treatments.
• DNA copy number variation analysis: Analyzes DNA copy number variations by qPCR.
• Multi-condition comparisons: Supports quick and reproducible comparisons of gene expression across experimental conditions and reference genes.

Methodology:

Click-qPCR accepts user-uploaded CSV data files and applies the ΔCq method for relative quantification, followed by ΔΔCq calculations for normalized expression values. Statistical significance is assessed using Welch's t-test for two-group comparisons or one-way ANOVA with Dunnett's post-hoc test for multiple group comparisons.