CLIMA 2

CLIMA 2 provides a molecular authentication database and identification resource for genetic authentication of human and animal cell lines across platforms.

Key Features:

• Integration with CLDB: Builds upon the Cell Line Data Base (CLDB) linking molecular authentication data to records for over 6,000 human and animal cell lines.
• Molecular Authentication Methods: Incorporates fingerprinting, single-locus short tandem repeat (STR) profiling, and single nucleotide polymorphism (SNP) validation for cell line identity verification.
• Hypertext Accessibility: Provides a hypertext version, HyperCLDB, that encodes internal links and supports retrieval via web spiders to navigate biological characteristics and authentication data.
• External Resource Links: Includes connections to external resources for cross-referencing and broader contextual information on cell lines.
• Reference Nomenclature: Contributes to establishing a reference nomenclature for standardized cell line identification.

Scientific Applications:

• Verification of experimental results: Confirms the identity of cell lines used in experiments.
• Reproducible research: Supports reproducibility through standardized authentication methods.
• Data integrity: Enhances integrity of datasets across studies involving human and animal cell lines.

Methodology:

Links molecular authentication data to specific cell line records using controlled vocabularies derived from international lists and taxonomies and encodes hypertext linkage (HyperCLDB) to enable web spider retrieval.