CLOOSE

CLOOSE standardizes, simplifies, and accelerates closed-loop experiments with functional imaging data through real-time data streaming and distributed computation.

Key Features:

• TCP/IP Interface: Interfaces with any frame-based imaging system via TCP/IP for real-time data streaming.
• Distributed Computation: Enables distributed computation for processing functional imaging data.
• Real-time Image Registration: Performs real-time image registration validated at imaging frequencies greater than or equal to 1 kHz.
• Signal Processing and Analysis: Provides real-time signal processing and analysis compatible with voltage indicators.
• Multiplane Imaging Support: Supports multiplane imaging in closed-loop neurofeedback paradigms.
• Online and Offline Z-tracking: Enables online and offline z-tracking for maintaining focal plane during imaging.
• Single Neuron to Population Dynamics: Supports neurofeedback paradigms ranging from single neuron to population dynamics.

Scientific Applications:

• Optical Brain-Computer Interfaces: Advances optical BCI experiments for precise manipulation and real-time readout of neuronal activity.
• Neurofeedback Paradigms: Supports neurofeedback experiments from single neuron to population dynamics, multiplane imaging, and z-tracking.
• Voltage Indicator Imaging: Enables closed-loop experiments with voltage indicators at imaging frequencies greater than or equal to 1 kHz.
• Clinical and Basic Neuroscience Research: Facilitates studies of neuronal activity in health and disease through closed-loop optical experimentation.

Methodology:

CLOOSE uses TCP/IP to interface with frame-based imaging systems for real-time data streaming and distributed computation; benchmark tests validate real-time accuracy in image registration, signal processing, and analysis.