CODA

CODA reconstructs three-dimensional tissue volumes at subcellular resolution from serially sectioned hematoxylin and eosin-stained tissue sections for quantitative analysis of microanatomical structures.

Key Features:

• Large-Scale Reconstruction: Handles tissue volumes up to multicentimeter cubed dimensions for whole-tissue structural studies.
• Subcellular Resolution: Achieves subcellular precision enabling analysis of microanatomical and subcellular structures.
• Quantifiable 3D Tissue Models: Produces quantifiable three-dimensional reconstructions amenable to structural analysis.
• Serial H&E Input: Operates on serially sectioned hematoxylin and eosin-stained tissue sections as input data.
• Multi-Structure Labeling: Supports labeling and analysis of multiple structures, demonstrated with ten distinct labeled structures.

Scientific Applications:

• Cross-tissue Microanatomy Reconstruction: Demonstrated reconstruction of distinct microanatomical structures in pancreas, skin, lung, and liver.
• Disease Progression and Tumorigenesis Analysis: Applied to study structural dynamics during disease progression and neoplastic transformation.
• Pancreatic Tumorigenesis Mapping: Used to examine the microanatomy of the human pancreas during tumorigenesis within the branching ductal system.
• Structural Heterogeneity and Morphology: Enabled investigation of heterogeneity and distinct 3D morphological phenotypes of precancerous lesions.
• Tumor Spread Pathways: Revealed pancreatic cancer tendency to spread along collagen fibers aligned with ductal, lobular, vascular, and neural structures.

Methodology:

Reconstruction proceeds from serial sectioning of hematoxylin and eosin-stained tissue sections followed by detailed three-dimensional reconstruction and labeling of structures.