colocr

colocr performs co-localization analysis of fluorescence microscopy images to quantify spatial distribution and assess potential functional or physical associations between two proteins within cells.

Key Features:

• Image loading: Supports loading fluorescence microscopy images for downstream analysis.
• Region of interest (ROI) selection: Enables selection and specification of ROIs for targeted co-localization assessment.
• Co-localization metrics computation: Calculates quantitative co-localization metrics to evaluate protein co-distribution.

Scientific Applications:

• Protein co-localization analysis: Quantifies spatial overlap of two proteins within cellular compartments using microscopy data.
• Inference of functional or physical associations: Supports interpretation of potential protein–protein interactions and cellular mechanisms from co-distribution patterns.

Methodology:

Computational steps explicitly include loading fluorescence microscopy images, selecting regions of interest (ROIs), and computing co-localization metrics to quantify protein co-distribution.