CompaCt

CompaCt performs automated integrative comparative analysis of complexome profiling datasets across multiple species to characterize and compare protein complexes using orthology information.

Key Features:

• Automated Integrative Analysis: Performs fully automated analysis of complexome profiling data derived from separation of intact protein complexes and mass spectrometric analysis of fractions.
• Comparative Approach: Handles datasets from multiple species to identify and compare conserved and taxon-specific components of protein complexes.
• Orthology Leveraging: Utilizes orthologous relationships between proteins across species to enhance comparative identification of conserved elements and taxon-specific features.

Scientific Applications:

• Characterization of Protein Complexes: Integrates multiple complexome profiles to improve characterization of protein complex composition and organization.
• Discovery of Novel Interactors and Complexes: Identifies novel candidate interactors and previously unrecognized protein complexes, including examples such as the emp24 complex, V-ATPase, and mitochondrial ATP synthase.
• Evolutionary Insights: Enables large-scale comparative studies that provide insights into the evolution of metazoan protein complexes, exemplified by analyses in Anopheles stephensi.

Methodology:

Applies integrative and comparative algorithms to complexome profiling datasets and identifies conserved and taxon-specific elements through orthology-based methods.