COMPSRA

COMPSRA performs comprehensive analysis of small RNA sequencing (RNA-Seq) data to identify, quantify, and characterize the full spectrum of small RNAs (typically <200 nucleotides) across biological samples.

Key Features:

• Comprehensive Analysis: Explores the entire small RNA'ome beyond microRNAs (miRNAs), including piRNAs, snRNAs, and snoRNAs, to enable broad identification and quantification of small RNA classes.
• Modular Design: Provides independent modules for quality control, alignment, annotation, microbe identification, and functional analysis that can operate separately or be integrated.
• Customizable Databases: Incorporates prebuilt customizable standard RNA databases to tailor small RNA identification and quantification to experimental requirements.
• Turnkey Sequence Processing: Includes sequence processing tools for end-to-end handling of small RNA sequencing data from raw reads to processed results.

Scientific Applications:

• Circulating RNA biomarker discovery: Enables detection and profiling of circulating small RNAs in serum for biomarker and diagnostic marker studies.
• Small RNA functional characterization: Supports exploration of small RNAs as functional molecules to study their roles in biological processes and disease.

Methodology:

Computational components explicitly include quality control, alignment, annotation, microbe identification, functional analysis, and sequence processing; the platform was evaluated on small RNA-seq data from serum samples of 12 healthy human controls and compared to existing tools, identifying greater diversity and abundance of small RNAs.