ConPlex

ConPlex analyzes evolutionary conservation of residues at protein interfaces to assess protein-protein interactions and functional sites within quaternary protein complexes.

Key Features:

• Interface Identification: Identifies protein interfaces within provided protein complex structures.
• Residue-Specific Conservation Analysis: Performs evolutionary conservation analysis on interface residues to identify conserved positions important for protein-protein interactions.
• Customizable Visualization: Maps conservation results onto the input protein complex structure for structural interpretation.

Scientific Applications:

• Determining Oligomerization States: Infers oligomeric states of proteins within complexes from patterns of sequence conservation.
• Characterizing Native Interfaces: Distinguishes native interfaces from non-specific crystal contacts using conservation signals.
• Discriminating Near-Native Structures: Aids in discriminating near-native structures from docking artifacts by comparing interface conservation.

Methodology:

Leverages evolutionary conservation by analyzing conservation of interface residues within protein complexes to provide insights into interaction stability and functionality.