Corna

Corna performs natural abundance correction (NAC) on metabolite intensity outputs from mass spectrometry to enable accurate quantification in stable isotope-based metabolomics.

Key Features:

• Natural Abundance Correction (NAC): Applies NAC to metabolite intensity outputs derived from mass spectrometry experiments, including stable isotope tracer studies.
• Multiple Tracer Support: Supports experiments with multiple tracer elements and includes correction capability for two tracer elements.
• Algorithm Validation: Implements algorithms that have been validated against published tools where applicable.
• Integration with Peak Integration Software: Integrates with an open-source peak integration software to process MS outputs and reduce overall processing time for stable isotope experiments.
• Resolution and MS Mode Support: Accommodates different instrument resolutions and tandem MS setups.

Scientific Applications:

• Metabolite quantification: Enables more accurate quantification of metabolites from mass spectrometry data in stable isotope experiments.
• Metabolite identification: Improves correction of isotopic patterns to support metabolite identification from MS data.
• Metabolic pathway discovery: Supports discovery of metabolic pathways by providing corrected isotopologue distributions for downstream analysis.
• Intracellular flux measurement: Facilitates measurement of intracellular fluxes using stable isotope labeling and mass spectrometry.

Methodology:

Applies validated computational algorithms for natural abundance correction to MS-derived metabolite intensities, supporting multiple tracer elements (including two tracers), different instrument resolutions, tandem MS, and integration with open-source peak integration software.