CorRelator

CorRelator performs correlation of fluorescence light microscopy (FLM) and cryo-electron microscopy (cryo-EM) data to enable spatial localization of fluorescently labeled proteins, macromolecular complexes, and organelles for cryo-correlative light and electron microscopy (cryo-CLEM).

Key Features:

• Flexibility in Workflow: Supports both on-the-fly and post-acquisition correlation schemes for integrating FLM and EM data.
• Adaptability Across Platforms: Operates with any configuration of fluorescence and transmission electron microscope (TEM) systems.
• Efficient Image Registration: Performs rapid image and position registration between FLM and TEM under cryogenic and ambient temperature conditions.
• Spatiotemporal Guidance: Leverages spatiotemporal cues from FLM to guide high-resolution structural investigations with cryo-EM.
• SerialEM Integration: Produces outputs compatible with SerialEM automated data collection to enable targeted investigations of transformed fluorescent targets of interest (TOIs) on registered EM images.

Scientific Applications:

• Structural Biology: Correlates FLM and cryo-EM to locate and characterize proteins and macromolecular complexes for high-resolution structural studies.
• Cell Biology: Enables mapping of organelles and the spatial organization and interactions of biomolecules within their cellular contexts using correlated FLM and EM data.
• Microscopy-driven Targeting: Facilitates targeted, microscopy-driven investigations that require precise image alignment and position registration across modalities.

Methodology:

Integrates image registration, transformation, and correlation processes between EM and FM (FLM/TEM) data, produces outputs compatible with SerialEM automated data collection, and has been benchmarked across various FLM and TEM instruments.