cpnDB

cpnDB provides a curated repository of cpn60 (chaperonin) gene sequences from bacteria, eukaryotes, and archaea to support phylogenetic analysis, organism identification, and studies of lateral gene transfer and codon usage.

Key Features:

• Comprehensive Data Collection: Approximately 2,000 records covering over 240 genera across bacteria, eukaryotes, and archaea, including sequences for both group I (Type I) and group II chaperonins.
• Universal PCR Target: Annotation of a 549–567-bp segment of the cpn60 coding region targeted by universal PCR primers for amplification and comparative analyses.
• Phylogenetic Utility: Curated cpn60 sequences enable phylogenetic studies and molecular identification across diverse taxa.

Scientific Applications:

• Clinical Studies: Provides cpn60 sequence targets for detecting and identifying pathogens in clinical research.
• Microbial Ecology: Supports analysis of community composition and interactions in environmental microbiome studies using cpn60-based profiling.
• Lateral Gene Transfer and Codon Usage Analysis: Enables investigation of lateral gene transfer events and codon usage variation using comprehensive cpn60 sequence data.

Methodology:

Constructed using open-source software tools.