Cpred

Cpred predicts viable circular permutation (CP) cleavage sites in proteins to assess positional feasibility prior to experimental implementation.

Key Features:

• CP site prediction: Predicts viable circular permutation (CP) cleavage sites within protein sequences.
• Positional feasibility assessment: Evaluates candidate positions for circular permutation to identify sites likely to yield viable permutants.
• Comprehensive predictive method: Implements a previously developed comprehensive method for predicting feasible CP cleavage sites.
• Impact evaluation: Provides computational evaluation of potential effects of CP on protein activity, stability, and functional properties.
• Experimental guidance: Generates predictions intended to guide selection of CP sites prior to experimental trials.

Scientific Applications:

• Protein engineering: Guides design of circularly permuted variants to explore altered activity and stability.
• Protein design and optimization: Assists selection of CP sites to optimize functional properties for engineering objectives.
• Functional diversification studies: Facilitates investigation of how terminal relocation via CP affects protein function and homology.
• Biotechnological applications: Supports computational evaluation of CP strategies for applied biotechnology research.

Methodology:

Implements the previously developed comprehensive computational method for predicting feasible circular permutation (CP) cleavage sites.