crispat

crispat facilitates the choice of a suitable guide assignment strategy for single-cell CRISPR screens by offering multiple assignment methods and means for their comparison.

Key Features:

• Multiple Assignment Methods: crispat offers 11 different guide assignment methods for single-cell CRISPR screens.
• Strategy Comparison: crispat provides functionality to compare different guide assignment strategies.

Scientific Applications:

• Guide Assignment Optimization: crispat identifies differences in the number of assigned cells, downregulation of target genes, and number of discoveries across different guide assignment strategies in single-cell CRISPR interference screens.
• Functional Genomics Analysis: crispat enables optimal power in single-cell CRISPR screens by facilitating selection of suitable guide assignment strategies for analyzing genetic intervention effects at scale.

Methodology:

crispat was demonstrated on four single-cell CRISPR interference screens at low multiplicity of infection from two studies.