CRISPR-Surfaceome

CRISPR-Surfaceome designs highly efficient single guide RNAs (sgRNAs) targeting human cell surface proteins for functional genomics, pathogen receptor identification, and drug target discovery.

Key Features:

• Targeted design for cell surface proteins: Tailors sgRNA design specifically to human cell surface proteins, including pathogen receptors and potential drug targets.
• Curated cell surface protein sequence database: Provides access to a curated database of cell surface protein sequences for candidate selection.
• sgRNA candidate generation: Generates multiple sgRNA candidates optimized for effective gene knockout.
• Off-target prediction and on-target optimization: Applies computational algorithms to predict off-target effects and enhance on-target activity.
• Experimental validation: Has been validated by experimental knockout studies on ICAM-1 achieving over 80% disruption.

Scientific Applications:

• Pathogen receptor identification: Enables interrogation of genes such as ICAM-1 to assess roles in pathogen entry, with ICAM-1 knockout cells showing resistance to rhinovirus infection.
• Drug target discovery: Facilitates exploration of cell surface proteins' roles in disease pathways to identify therapeutic targets.
• Functional genomics studies: Supports investigations into the biological functions and interactions of cell surface proteins via targeted knockout.

Methodology:

Generates multiple sgRNA candidates from target gene input and applies computational algorithms to predict off-target effects and optimize on-target activity.